The structure of C hordein

J. M. Field; A. S. Tatham; A. M. Baker; P. R. Shewry

doi:10.1016/0014-5793(86)80514-2

The structure of C hordein

J. M. Field^*, A. S. Tatham, A. M. Baker, P. R. Shewry

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

32 Citations (Scopus)

Abstract

The intrinsic viscosity of C hordein was determined in 70% ( v v) aqueous ethanol (± 10 mM NaCl) and 0.1 M acetic acid at temperatures between 15.3 and 50°C. Calculations based on the assumption (supported by previously published results) that C hordein has an ordered conformation indicated that the molecule is rod shaped, with molecular dimensions varying from about 360 × 17 Å to 265 × 20 Å. Circular dichroism spectroscopy indicated a secondary structure rich in β-turns, with some conformational differences in the two solvents and at different temperatures. The results are compatible with C hordein having a helical secondary structure based on repetitive β-turns.

Original language	English
Pages (from-to)	76-80
Number of pages	5
Journal	FEBS Letters
Volume	200
Issue number	1
DOIs	https://doi.org/10.1016/0014-5793(86)80514-2
Publication status	Published - 5 May 1986
Externally published	Yes

Keywords

Hordein Molecular dimensions Secondary structure β-turn

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title = "The structure of C hordein",

abstract = "The intrinsic viscosity of C hordein was determined in 70\% ( v v) aqueous ethanol (± 10 mM NaCl) and 0.1 M acetic acid at temperatures between 15.3 and 50°C. Calculations based on the assumption (supported by previously published results) that C hordein has an ordered conformation indicated that the molecule is rod shaped, with molecular dimensions varying from about 360 × 17 {\AA} to 265 × 20 {\AA}. Circular dichroism spectroscopy indicated a secondary structure rich in β-turns, with some conformational differences in the two solvents and at different temperatures. The results are compatible with C hordein having a helical secondary structure based on repetitive β-turns.",

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T1 - The structure of C hordein

AU - Field, J. M.

AU - Tatham, A. S.

AU - Baker, A. M.

AU - Shewry, P. R.

PY - 1986/5/5

Y1 - 1986/5/5

N2 - The intrinsic viscosity of C hordein was determined in 70% ( v v) aqueous ethanol (± 10 mM NaCl) and 0.1 M acetic acid at temperatures between 15.3 and 50°C. Calculations based on the assumption (supported by previously published results) that C hordein has an ordered conformation indicated that the molecule is rod shaped, with molecular dimensions varying from about 360 × 17 Å to 265 × 20 Å. Circular dichroism spectroscopy indicated a secondary structure rich in β-turns, with some conformational differences in the two solvents and at different temperatures. The results are compatible with C hordein having a helical secondary structure based on repetitive β-turns.

AB - The intrinsic viscosity of C hordein was determined in 70% ( v v) aqueous ethanol (± 10 mM NaCl) and 0.1 M acetic acid at temperatures between 15.3 and 50°C. Calculations based on the assumption (supported by previously published results) that C hordein has an ordered conformation indicated that the molecule is rod shaped, with molecular dimensions varying from about 360 × 17 Å to 265 × 20 Å. Circular dichroism spectroscopy indicated a secondary structure rich in β-turns, with some conformational differences in the two solvents and at different temperatures. The results are compatible with C hordein having a helical secondary structure based on repetitive β-turns.

KW - Hordein Molecular dimensions Secondary structure β-turn

UR - https://www.scopus.com/pages/publications/0022483222

U2 - 10.1016/0014-5793(86)80514-2

DO - 10.1016/0014-5793(86)80514-2

M3 - Article

AN - SCOPUS:0022483222

SN - 0014-5793

VL - 200

SP - 76

EP - 80

JO - FEBS Letters

JF - FEBS Letters

IS - 1

ER -

The structure of C hordein

Abstract

Keywords

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