TY - JOUR
T1 - Structural characterization of a methionine-rich, emulsifying protein from sunflower seed
AU - Pandya, Maya J.
AU - Sessions, Richard B.
AU - Williams, Phil B.
AU - Dempsey, Christopher E.
AU - Tatham, Arthur S.
AU - Shewry, Peter R.
AU - Clarke, Anthony R.
PY - 2000/2/15
Y1 - 2000/2/15
N2 - The 2 S seed storage protein, sunflower albumin 8, contains an unusually high proportion of hydrophobic residues including 16 methionines in a mature protein of 103 amino acids. A structural model, based on the known structure of a related protein, has been constructed as a four-helix bundle cross- linked by four disulphide bonds. This model structure is consistent with data from circular dichroism and nuclear magnetic resonance experiments. Analysis of the model's surface shows the presence of a large hydrophobic face that may be responsible for the highly stable emulsions this protein is known to form with oil/water mixtures. (C) 2000 Wiley-Liss, Inc.
AB - The 2 S seed storage protein, sunflower albumin 8, contains an unusually high proportion of hydrophobic residues including 16 methionines in a mature protein of 103 amino acids. A structural model, based on the known structure of a related protein, has been constructed as a four-helix bundle cross- linked by four disulphide bonds. This model structure is consistent with data from circular dichroism and nuclear magnetic resonance experiments. Analysis of the model's surface shows the presence of a large hydrophobic face that may be responsible for the highly stable emulsions this protein is known to form with oil/water mixtures. (C) 2000 Wiley-Liss, Inc.
KW - Emulsifying agent
KW - Homology model
KW - Hydrophobic face
KW - Methionine-rich
KW - Seed storage protein
UR - http://www.scopus.com/inward/record.url?scp=0034651674&partnerID=8YFLogxK
U2 - 10.1002/(SICI)1097-0134(20000215)38:3<341::AID-PROT9>3.0.CO;2-D
DO - 10.1002/(SICI)1097-0134(20000215)38:3<341::AID-PROT9>3.0.CO;2-D
M3 - Article
C2 - 10713993
AN - SCOPUS:0034651674
SN - 0887-3585
VL - 38
SP - 341
EP - 349
JO - Proteins: Structure, Function and Genetics
JF - Proteins: Structure, Function and Genetics
IS - 3
ER -