Abstract
Oxygen is an essential substrate for mitochondrial respiration and many essential synthetic and degradative reactions and it is an essential component in oxidative damage. Its supply to tissue and cells is therefore a critical parameter
governing normal homeostasis. It is hypothesized that oxygen sensing and control
of vessel tone are closely linked and controlled. Nitric oxide (NO) is an important
regulator of vascular tone, functioning as a potent vasodilator when produced by
endothelial cells lining the blood vessels. Nitric oxide synthases (NOS) release
NO from the endothelial cells in response to a variety of stimuli, e.g., agonists
(such as bradykinin or acetyl choline), shear stress, and flow. Oxygen is a
critical cofactor for NO biosynthesis and is required at concentrations above 9/zM
for normal NO production,
governing normal homeostasis. It is hypothesized that oxygen sensing and control
of vessel tone are closely linked and controlled. Nitric oxide (NO) is an important
regulator of vascular tone, functioning as a potent vasodilator when produced by
endothelial cells lining the blood vessels. Nitric oxide synthases (NOS) release
NO from the endothelial cells in response to a variety of stimuli, e.g., agonists
(such as bradykinin or acetyl choline), shear stress, and flow. Oxygen is a
critical cofactor for NO biosynthesis and is required at concentrations above 9/zM
for normal NO production,
| Original language | English |
|---|---|
| Pages (from-to) | 52-66 |
| Number of pages | 15 |
| Journal | Methods in Enzymology |
| Volume | 359 |
| DOIs | |
| Publication status | Published - 2002 |
| Externally published | Yes |