TY - JOUR
T1 - Quantitative temporal viromics
T2 - An approach to investigate host-pathogen interaction
AU - Weekes, Michael P.
AU - Tomasec, Peter
AU - Huttlin, Edward L.
AU - Fielding, Ceri A.
AU - Nusinow, David
AU - Stanton, Richard J.
AU - Wang, Eddie C.Y.
AU - Aicheler, Rebecca
AU - Murrell, Isa
AU - Wilkinson, Gavin W.G.
AU - Lehner, Paul J.
AU - Gygi, Steven P.
PY - 2014/6/5
Y1 - 2014/6/5
N2 - A systematic quantitative analysis of temporal changes in host and viral proteins throughout the course of a productive infection could provide dynamic insights into virus-host interaction. We developed a proteomic technique called "quantitative temporal viromics" (QTV), which employs multiplexed tandem-mass-tag-based mass spectrometry. Human cytomegalovirus (HCMV) is not only an important pathogen but a paradigm of viral immune evasion. QTV detailed how HCMV orchestrates the expression of >8,000 cellular proteins, including 1,200 cell-surface proteins to manipulate signaling pathways and counterintrinsic, innate, and adaptive immune defenses. QTV predicted natural killer and T cell ligands, as well as 29 viral proteins present at the cell surface, potential therapeutic targets. Temporal profiles of >80% of HCMV canonical genes and 14 noncanonical HCMV open reading frames were defined. QTV is a powerful method that can yield important insights into viral infection and is applicable to any virus with a robust in vitro model. PaperClip
AB - A systematic quantitative analysis of temporal changes in host and viral proteins throughout the course of a productive infection could provide dynamic insights into virus-host interaction. We developed a proteomic technique called "quantitative temporal viromics" (QTV), which employs multiplexed tandem-mass-tag-based mass spectrometry. Human cytomegalovirus (HCMV) is not only an important pathogen but a paradigm of viral immune evasion. QTV detailed how HCMV orchestrates the expression of >8,000 cellular proteins, including 1,200 cell-surface proteins to manipulate signaling pathways and counterintrinsic, innate, and adaptive immune defenses. QTV predicted natural killer and T cell ligands, as well as 29 viral proteins present at the cell surface, potential therapeutic targets. Temporal profiles of >80% of HCMV canonical genes and 14 noncanonical HCMV open reading frames were defined. QTV is a powerful method that can yield important insights into viral infection and is applicable to any virus with a robust in vitro model. PaperClip
UR - https://www.scopus.com/pages/publications/84902108855
U2 - 10.1016/j.cell.2014.04.028
DO - 10.1016/j.cell.2014.04.028
M3 - Article
C2 - 24906157
AN - SCOPUS:84902108855
SN - 0092-8674
VL - 157
SP - 1460
EP - 1472
JO - Cell
JF - Cell
IS - 6
ER -