TY - JOUR
T1 - Proliferation and interleukin 5 production by CD8hiCD57+ T cells
AU - Chong, Lee K.
AU - Aicheler, Rebecca J.
AU - Llewellyn-Lacey, Sian
AU - Tomasec, Peter
AU - Brennan, Paul
AU - Wang, Eddie C.Y.
PY - 2008/4/7
Y1 - 2008/4/7
N2 - CD8hiCD57+T cells have previously been described as effector memory T cells with minimal expansion capacity and high susceptibility to activation-induced cell death. In contrast, we demonstrate here that CD8hiCD57+ T cells are capable of rapid expansion using multiple techniques including [3H]thymidine uptake, flow cytometric bead-based enumeration and standard haemocytometer counting. Previous reports can be explained by marked inhibition of activation-induced expansion and increased 7-amino-actinomycin D uptake by CD8hiCD57+ T cells following treatment with CFSE, a dye previously used to measure their proliferation, combined with specific media requirements for the growth of this cell subset. The ability of CD8hiCD57+ T cells to further differentiate is highlighted by a distinct cytokine profile late after activation that includes the unexpected release of high levels of interleukin 5. These data indicate that CD8hiCD57+ T cells should not be considered as "end-stage" effector T cells incapable of proliferation, but represent a highly differentiated subset capable of rapid division and exhibiting novel functions separate from their previously described cytotoxic and IFN-γ responses.
AB - CD8hiCD57+T cells have previously been described as effector memory T cells with minimal expansion capacity and high susceptibility to activation-induced cell death. In contrast, we demonstrate here that CD8hiCD57+ T cells are capable of rapid expansion using multiple techniques including [3H]thymidine uptake, flow cytometric bead-based enumeration and standard haemocytometer counting. Previous reports can be explained by marked inhibition of activation-induced expansion and increased 7-amino-actinomycin D uptake by CD8hiCD57+ T cells following treatment with CFSE, a dye previously used to measure their proliferation, combined with specific media requirements for the growth of this cell subset. The ability of CD8hiCD57+ T cells to further differentiate is highlighted by a distinct cytokine profile late after activation that includes the unexpected release of high levels of interleukin 5. These data indicate that CD8hiCD57+ T cells should not be considered as "end-stage" effector T cells incapable of proliferation, but represent a highly differentiated subset capable of rapid division and exhibiting novel functions separate from their previously described cytotoxic and IFN-γ responses.
KW - CD57
KW - CD8 T cells
KW - IL-5
UR - http://www.scopus.com/inward/record.url?scp=44849083567&partnerID=8YFLogxK
U2 - 10.1002/eji.200737687
DO - 10.1002/eji.200737687
M3 - Article
C2 - 18383036
AN - SCOPUS:44849083567
SN - 0014-2980
VL - 38
SP - 995
EP - 1000
JO - European Journal of Immunology
JF - European Journal of Immunology
IS - 4
ER -