Megakaryocytic differentiation is accompanied by a reduction in cell migratory potential

Anthony Mathur, Ying Hong, John F. Martin, Jorge D. Erusalimsky*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

8 Citations (Scopus)

Abstract

Megakaryocytes (MKs) have been found in the peripheral circulation, suggesting that they can migrate out of the bone marrow. In order to evaluate if megakaryocytic differentiation confers a migratory phenotype, we investigated this property in the haematopoietic cell lines MO7e and UT-7/mpl and in CD34+ progenitor cells before and after induction of differentiation by thrombopoietin (TPO). Migration was studied using a bicompartmental culture system in the presence or absence of a bone marrow endothelial cell monolayer. Preincubation with TPO led to a significant reduction in stromal cell-derived factor-1 (SDF-1)-induced migration of MO7e cells (0.7% ± 0.08% for TPO-treated vs. 2.6% ± 0.3% for controls P < 0.05). A similar decreased migratory response was seen with UT-7/mpl cells (7.4% ± 0.4% for TPO-treated vs. 11.1% ± 0.01% for controls, P < 0.05), although these cells did not migrate in response to SDF-1. CD34+ cells partially differentiated with TPO showed decreased migration following further TPO-induced maturation (13.9% ± 1.8% for TPO-treated vs. 24.1% ± 1.8% for untreated, P < 0.05). This reduction was more pronounced in the large MK (≥ 4n) fraction. These results demonstrate that megakaryocytic differentiation is accompanied by a partial suppression of the haematopoietic cell migratory phenotype.

Original languageEnglish
Pages (from-to)459-465
Number of pages7
JournalBritish Journal of Haematology
Volume112
Issue number2
DOIs
Publication statusPublished - 2001
Externally publishedYes

Keywords

  • Bone marrow
  • Haematopoeisis
  • Megakaryocyte
  • Thrombopoietin
  • Transendothelial migration

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