TY - JOUR
T1 - Megakaryocytic differentiation is accompanied by a reduction in cell migratory potential
AU - Mathur, Anthony
AU - Hong, Ying
AU - Martin, John F.
AU - Erusalimsky, Jorge D.
PY - 2001
Y1 - 2001
N2 - Megakaryocytes (MKs) have been found in the peripheral circulation, suggesting that they can migrate out of the bone marrow. In order to evaluate if megakaryocytic differentiation confers a migratory phenotype, we investigated this property in the haematopoietic cell lines MO7e and UT-7/mpl and in CD34+ progenitor cells before and after induction of differentiation by thrombopoietin (TPO). Migration was studied using a bicompartmental culture system in the presence or absence of a bone marrow endothelial cell monolayer. Preincubation with TPO led to a significant reduction in stromal cell-derived factor-1 (SDF-1)-induced migration of MO7e cells (0.7% ± 0.08% for TPO-treated vs. 2.6% ± 0.3% for controls P < 0.05). A similar decreased migratory response was seen with UT-7/mpl cells (7.4% ± 0.4% for TPO-treated vs. 11.1% ± 0.01% for controls, P < 0.05), although these cells did not migrate in response to SDF-1. CD34+ cells partially differentiated with TPO showed decreased migration following further TPO-induced maturation (13.9% ± 1.8% for TPO-treated vs. 24.1% ± 1.8% for untreated, P < 0.05). This reduction was more pronounced in the large MK (≥ 4n) fraction. These results demonstrate that megakaryocytic differentiation is accompanied by a partial suppression of the haematopoietic cell migratory phenotype.
AB - Megakaryocytes (MKs) have been found in the peripheral circulation, suggesting that they can migrate out of the bone marrow. In order to evaluate if megakaryocytic differentiation confers a migratory phenotype, we investigated this property in the haematopoietic cell lines MO7e and UT-7/mpl and in CD34+ progenitor cells before and after induction of differentiation by thrombopoietin (TPO). Migration was studied using a bicompartmental culture system in the presence or absence of a bone marrow endothelial cell monolayer. Preincubation with TPO led to a significant reduction in stromal cell-derived factor-1 (SDF-1)-induced migration of MO7e cells (0.7% ± 0.08% for TPO-treated vs. 2.6% ± 0.3% for controls P < 0.05). A similar decreased migratory response was seen with UT-7/mpl cells (7.4% ± 0.4% for TPO-treated vs. 11.1% ± 0.01% for controls, P < 0.05), although these cells did not migrate in response to SDF-1. CD34+ cells partially differentiated with TPO showed decreased migration following further TPO-induced maturation (13.9% ± 1.8% for TPO-treated vs. 24.1% ± 1.8% for untreated, P < 0.05). This reduction was more pronounced in the large MK (≥ 4n) fraction. These results demonstrate that megakaryocytic differentiation is accompanied by a partial suppression of the haematopoietic cell migratory phenotype.
KW - Bone marrow
KW - Haematopoeisis
KW - Megakaryocyte
KW - Thrombopoietin
KW - Transendothelial migration
UR - http://www.scopus.com/inward/record.url?scp=0035124054&partnerID=8YFLogxK
U2 - 10.1046/j.1365-2141.2001.02534.x
DO - 10.1046/j.1365-2141.2001.02534.x
M3 - Article
C2 - 11167847
AN - SCOPUS:0035124054
SN - 0007-1048
VL - 112
SP - 459
EP - 465
JO - British Journal of Haematology
JF - British Journal of Haematology
IS - 2
ER -