Increased monocyte actin polymerization in rat blood after intratracheal instillation of air pollution particles

Rachel A. Adams; Ameena Al-Mosawi; Kelly Bérubé; Tim Jones; Timothy Higgins; Shelley Ann Evans

doi:10.3233/BIR-14027

Increased monocyte actin polymerization in rat blood after intratracheal instillation of air pollution particles

Rachel A. Adams^*, Ameena Al-Mosawi, Kelly Bérubé, Tim Jones, Timothy Higgins, Shelley Ann Evans

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

5 Citations (Scopus)

Abstract

BACKGROUND: Exposure to particulate air pollution is associated with an increased risk of cardiovascular disease. The mechanism by which exposure increases risk is poorly understood but could involve changes in the flow properties of blood.

OBJECTIVE: The aim of this investigation was to assess the effect, in rats, of intratracheal instillation of particulate air pollution on leukocyte flow properties by measurement of polymorphonucleocyte (PMN) and monocyte actin polymerisation.

METHODS: Rats were exposed to particulate air pollution by intratracheal instillation of PM₁₀. Blood was collected from test and control animals at 3 days (n=10) and 6 weeks (n=10) after dust instillation. Partial differential leukocyte counts were performed. The intracellular F-actin content of blood PMNs and monocytes was determined by staining with FITC-phalloidin and flow cytometric determination of mean florescence intensity (MFI).

RESULTS: There were no significant changes in PMN MFI (p=0.369, ANOVA) or cell counts (p=0.753, ANOVA). There was a significant increase in monocyte MFI (p=0.004, ANOVA) and a decrease in monocyte cell count (p=0.003, ANOVA) in instilled rats.

CONCLUSIONS: Intratracheal instillation of air pollution particles resulted in an increase in blood monocyte actin polymerisation, which may cause trapping of monocytes. This could be a mechanism by which exposure to air pollution increases the risk of cardiovascular disease.

Original language	English
Pages (from-to)	329-338
Number of pages	10
Journal	Biorheology
Volume	51
Issue number	4-5
DOIs	https://doi.org/10.3233/BIR-14027
Publication status	Published - Sept 2014

Keywords

Particles
cardiovascular disease
cytoskeleton
leukocyte
neutrophil

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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Cite this

@article{37264b2ea8d74862b81b2e91d7be64fd,

title = "Increased monocyte actin polymerization in rat blood after intratracheal instillation of air pollution particles",

abstract = "BACKGROUND: Exposure to particulate air pollution is associated with an increased risk of cardiovascular disease. The mechanism by which exposure increases risk is poorly understood but could involve changes in the flow properties of blood.OBJECTIVE: The aim of this investigation was to assess the effect, in rats, of intratracheal instillation of particulate air pollution on leukocyte flow properties by measurement of polymorphonucleocyte (PMN) and monocyte actin polymerisation.METHODS: Rats were exposed to particulate air pollution by intratracheal instillation of PM10. Blood was collected from test and control animals at 3 days (n=10) and 6 weeks (n=10) after dust instillation. Partial differential leukocyte counts were performed. The intracellular F-actin content of blood PMNs and monocytes was determined by staining with FITC-phalloidin and flow cytometric determination of mean florescence intensity (MFI).RESULTS: There were no significant changes in PMN MFI (p=0.369, ANOVA) or cell counts (p=0.753, ANOVA). There was a significant increase in monocyte MFI (p=0.004, ANOVA) and a decrease in monocyte cell count (p=0.003, ANOVA) in instilled rats.CONCLUSIONS: Intratracheal instillation of air pollution particles resulted in an increase in blood monocyte actin polymerisation, which may cause trapping of monocytes. This could be a mechanism by which exposure to air pollution increases the risk of cardiovascular disease.",

keywords = "Particles, cardiovascular disease, cytoskeleton, leukocyte, neutrophil",

author = "Adams, {Rachel A.} and Ameena Al-Mosawi and Kelly B{\'e}rub{\'e} and Tim Jones and Timothy Higgins and Evans, {Shelley Ann}",

note = "Publisher Copyright: {\textcopyright} 2014-IOS Press and the authors.",

year = "2014",

month = sep,

doi = "10.3233/BIR-14027",

language = "English",

volume = "51",

pages = "329--338",

journal = "Biorheology",

issn = "0006-355X",

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TY - JOUR

T1 - Increased monocyte actin polymerization in rat blood after intratracheal instillation of air pollution particles

AU - Adams, Rachel A.

AU - Al-Mosawi, Ameena

AU - Bérubé, Kelly

AU - Jones, Tim

AU - Higgins, Timothy

AU - Evans, Shelley Ann

PY - 2014/9

Y1 - 2014/9

N2 - BACKGROUND: Exposure to particulate air pollution is associated with an increased risk of cardiovascular disease. The mechanism by which exposure increases risk is poorly understood but could involve changes in the flow properties of blood.OBJECTIVE: The aim of this investigation was to assess the effect, in rats, of intratracheal instillation of particulate air pollution on leukocyte flow properties by measurement of polymorphonucleocyte (PMN) and monocyte actin polymerisation.METHODS: Rats were exposed to particulate air pollution by intratracheal instillation of PM10. Blood was collected from test and control animals at 3 days (n=10) and 6 weeks (n=10) after dust instillation. Partial differential leukocyte counts were performed. The intracellular F-actin content of blood PMNs and monocytes was determined by staining with FITC-phalloidin and flow cytometric determination of mean florescence intensity (MFI).RESULTS: There were no significant changes in PMN MFI (p=0.369, ANOVA) or cell counts (p=0.753, ANOVA). There was a significant increase in monocyte MFI (p=0.004, ANOVA) and a decrease in monocyte cell count (p=0.003, ANOVA) in instilled rats.CONCLUSIONS: Intratracheal instillation of air pollution particles resulted in an increase in blood monocyte actin polymerisation, which may cause trapping of monocytes. This could be a mechanism by which exposure to air pollution increases the risk of cardiovascular disease.

AB - BACKGROUND: Exposure to particulate air pollution is associated with an increased risk of cardiovascular disease. The mechanism by which exposure increases risk is poorly understood but could involve changes in the flow properties of blood.OBJECTIVE: The aim of this investigation was to assess the effect, in rats, of intratracheal instillation of particulate air pollution on leukocyte flow properties by measurement of polymorphonucleocyte (PMN) and monocyte actin polymerisation.METHODS: Rats were exposed to particulate air pollution by intratracheal instillation of PM10. Blood was collected from test and control animals at 3 days (n=10) and 6 weeks (n=10) after dust instillation. Partial differential leukocyte counts were performed. The intracellular F-actin content of blood PMNs and monocytes was determined by staining with FITC-phalloidin and flow cytometric determination of mean florescence intensity (MFI).RESULTS: There were no significant changes in PMN MFI (p=0.369, ANOVA) or cell counts (p=0.753, ANOVA). There was a significant increase in monocyte MFI (p=0.004, ANOVA) and a decrease in monocyte cell count (p=0.003, ANOVA) in instilled rats.CONCLUSIONS: Intratracheal instillation of air pollution particles resulted in an increase in blood monocyte actin polymerisation, which may cause trapping of monocytes. This could be a mechanism by which exposure to air pollution increases the risk of cardiovascular disease.

KW - Particles

KW - cardiovascular disease

KW - cytoskeleton

KW - leukocyte

KW - neutrophil

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U2 - 10.3233/BIR-14027

DO - 10.3233/BIR-14027

M3 - Article

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SN - 0006-355X

VL - 51

SP - 329

EP - 338

JO - Biorheology

JF - Biorheology

IS - 4-5

ER -

Increased monocyte actin polymerization in rat blood after intratracheal instillation of air pollution particles

Abstract

Keywords

UN SDGs

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