Effects of E. Coli 0111.b4 lipopolysaccharide on spin-labelled murine macrophage and hepatocyte membranes

S. K. Jackson; P. E. James; C. C. Rowlands; J. C. Evans

doi:10.3109/10715768909087971

Effects of E. Coli 0111.b4 lipopolysaccharide on spin-labelled murine macrophage and hepatocyte membranes

S. K. Jackson^*, P. E. James, C. C. Rowlands, J. C. Evans

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

8 Citations (Scopus)

Abstract

Macrophages and hepatocytes from normal and BCG-primed mice have been spin-labelled in their membranes with 5- and 16-doxyl stearic acid. Incubation of spin-labelled cells from BCG-primed animals with lipopolysaccharide from E. coli 0111 B4 produced a detectable and transient disturbance in the cell membranes as reflected by an increase in the order parameter measured from the electron spin resonance spectra of 5-doxyl-stearate. This membrane disturbance was maximal at 3-4 hours of incubation and was only detected with cells from mice primed with BCG. Spectra obtained from the 16-doxyl-stearate-labelled cells showed no change in order parameter on incubation with lipopolysaccharide.

Original language	English
Pages (from-to)	47-53
Number of pages	7
Journal	Free Radical Research
Volume	8
Issue number	1
DOIs	https://doi.org/10.3109/10715768909087971
Publication status	Published - 1989
Externally published	Yes

Keywords

Electron spin resonance
Lipopolysaccharide
Macrophage. hepatocyte
Spin label

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title = "Effects of E. Coli 0111.b4 lipopolysaccharide on spin-labelled murine macrophage and hepatocyte membranes",

abstract = "Macrophages and hepatocytes from normal and BCG-primed mice have been spin-labelled in their membranes with 5- and 16-doxyl stearic acid. Incubation of spin-labelled cells from BCG-primed animals with lipopolysaccharide from E. coli 0111 B4 produced a detectable and transient disturbance in the cell membranes as reflected by an increase in the order parameter measured from the electron spin resonance spectra of 5-doxyl-stearate. This membrane disturbance was maximal at 3-4 hours of incubation and was only detected with cells from mice primed with BCG. Spectra obtained from the 16-doxyl-stearate-labelled cells showed no change in order parameter on incubation with lipopolysaccharide.",

keywords = "Electron spin resonance, Lipopolysaccharide, Macrophage. hepatocyte, Spin label",

author = "Jackson, {S. K.} and James, {P. E.} and Rowlands, {C. C.} and Evans, {J. C.}",

year = "1989",

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T1 - Effects of E. Coli 0111.b4 lipopolysaccharide on spin-labelled murine macrophage and hepatocyte membranes

AU - Jackson, S. K.

AU - James, P. E.

AU - Rowlands, C. C.

AU - Evans, J. C.

PY - 1989

Y1 - 1989

N2 - Macrophages and hepatocytes from normal and BCG-primed mice have been spin-labelled in their membranes with 5- and 16-doxyl stearic acid. Incubation of spin-labelled cells from BCG-primed animals with lipopolysaccharide from E. coli 0111 B4 produced a detectable and transient disturbance in the cell membranes as reflected by an increase in the order parameter measured from the electron spin resonance spectra of 5-doxyl-stearate. This membrane disturbance was maximal at 3-4 hours of incubation and was only detected with cells from mice primed with BCG. Spectra obtained from the 16-doxyl-stearate-labelled cells showed no change in order parameter on incubation with lipopolysaccharide.

AB - Macrophages and hepatocytes from normal and BCG-primed mice have been spin-labelled in their membranes with 5- and 16-doxyl stearic acid. Incubation of spin-labelled cells from BCG-primed animals with lipopolysaccharide from E. coli 0111 B4 produced a detectable and transient disturbance in the cell membranes as reflected by an increase in the order parameter measured from the electron spin resonance spectra of 5-doxyl-stearate. This membrane disturbance was maximal at 3-4 hours of incubation and was only detected with cells from mice primed with BCG. Spectra obtained from the 16-doxyl-stearate-labelled cells showed no change in order parameter on incubation with lipopolysaccharide.

KW - Electron spin resonance

KW - Lipopolysaccharide

KW - Macrophage. hepatocyte

KW - Spin label

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DO - 10.3109/10715768909087971

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SN - 1071-5762

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JO - Free Radical Research

JF - Free Radical Research

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ER -

Effects of E. Coli 0111.b4 lipopolysaccharide on spin-labelled murine macrophage and hepatocyte membranes

Abstract

Keywords

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