Characterization of a sunflower seed albumin which associates with oil bodies

Patrick J.E. Thoyts; Johnathan A. Napier; Mark Millichip; A. Keith Stobart; W. Trevor Griffiths; Arthur S. Tatham; Peter R. Shewry

doi:10.1016/0168-9452(96)04429-9

Characterization of a sunflower seed albumin which associates with oil bodies

Patrick J.E. Thoyts, Johnathan A. Napier, Mark Millichip, A. Keith Stobart, W. Trevor Griffiths, Arthur S. Tatham, Peter R. Shewry^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

16 Citations (Scopus)

Abstract

An M(r) 22 500 protein was purified from isolated oil bodies from sunflower cotyledons. N-terminal amino acid sequencing showed that this protein belonged to the 2S albumin group of storage proteins. A corresponding cDNA clone encoded a preproprotein, comprising two mature 2S albumin proteins one of which corresponded to the oil-body associated albumin. In contrast, the second albumin encoded by the eDNA was related to components purified from a total albumin fraction by RP-HPLC.

Original language	English
Pages (from-to)	119-125
Number of pages	7
Journal	Plant Science
Volume	118
Issue number	2
DOIs	https://doi.org/10.1016/0168-9452(96)04429-9
Publication status	Published - 16 Aug 1996
Externally published	Yes

Keywords

2S albumins
Oil bodies
Proteins
Seeds
Sunflower

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10.1016/0168-9452(96)04429-9

Cite this

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title = "Characterization of a sunflower seed albumin which associates with oil bodies",

abstract = "An M(r) 22 500 protein was purified from isolated oil bodies from sunflower cotyledons. N-terminal amino acid sequencing showed that this protein belonged to the 2S albumin group of storage proteins. A corresponding cDNA clone encoded a preproprotein, comprising two mature 2S albumin proteins one of which corresponded to the oil-body associated albumin. In contrast, the second albumin encoded by the eDNA was related to components purified from a total albumin fraction by RP-HPLC.",

keywords = "2S albumins, Oil bodies, Proteins, Seeds, Sunflower",

author = "Thoyts, \{Patrick J.E.\} and Napier, \{Johnathan A.\} and Mark Millichip and Stobart, \{A. Keith\} and Griffiths, \{W. Trevor\} and Tatham, \{Arthur S.\} and Shewry, \{Peter R.\}",

year = "1996",

month = aug,

day = "16",

doi = "10.1016/0168-9452(96)04429-9",

language = "English",

volume = "118",

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journal = "Plant Science",

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TY - JOUR

T1 - Characterization of a sunflower seed albumin which associates with oil bodies

AU - Thoyts, Patrick J.E.

AU - Napier, Johnathan A.

AU - Millichip, Mark

AU - Stobart, A. Keith

AU - Griffiths, W. Trevor

AU - Tatham, Arthur S.

AU - Shewry, Peter R.

PY - 1996/8/16

Y1 - 1996/8/16

N2 - An M(r) 22 500 protein was purified from isolated oil bodies from sunflower cotyledons. N-terminal amino acid sequencing showed that this protein belonged to the 2S albumin group of storage proteins. A corresponding cDNA clone encoded a preproprotein, comprising two mature 2S albumin proteins one of which corresponded to the oil-body associated albumin. In contrast, the second albumin encoded by the eDNA was related to components purified from a total albumin fraction by RP-HPLC.

AB - An M(r) 22 500 protein was purified from isolated oil bodies from sunflower cotyledons. N-terminal amino acid sequencing showed that this protein belonged to the 2S albumin group of storage proteins. A corresponding cDNA clone encoded a preproprotein, comprising two mature 2S albumin proteins one of which corresponded to the oil-body associated albumin. In contrast, the second albumin encoded by the eDNA was related to components purified from a total albumin fraction by RP-HPLC.

KW - 2S albumins

KW - Oil bodies

KW - Proteins

KW - Seeds

KW - Sunflower

UR - https://www.scopus.com/pages/publications/0030590145

U2 - 10.1016/0168-9452(96)04429-9

DO - 10.1016/0168-9452(96)04429-9

M3 - Article

AN - SCOPUS:0030590145

SN - 0168-9452

VL - 118

SP - 119

EP - 125

JO - Plant Science

JF - Plant Science

IS - 2

ER -

Characterization of a sunflower seed albumin which associates with oil bodies

Abstract

Keywords

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