Characterization of a panel of monoclonal anti-gliadin antibodies

E. N. Clare Mills; Shirley R. Burgess; Arthur S. Tatham; Peter R. Shewry; Henry W.S. Chan; Michael R.A. Morgan

doi:10.1016/S0733-5210(09)80113-6

Characterization of a panel of monoclonal anti-gliadin antibodies

E. N. Clare Mills^*, Shirley R. Burgess, Arthur S. Tatham, Peter R. Shewry, Henry W.S. Chan, Michael R.A. Morgan

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

21 Citations (Scopus)

Abstract

A panel of monoclonal antibodies has been raised against a total gliadin fraction prepared from the hard bread wheat cultivar Avalon. The antibodies have been characterized by enzyme-linked immunosorhent assay and immunoblotting techniques for their interactions with a range of cereal prolamins, purified gliadins and short synthetic peptides. The antibodies showed broad, intermediate and narrow specificities. The immunological approach offers the protein chemist an additional means of studying the structures and interrelationships of cereal proteins.

Original language	English
Pages (from-to)	89-101
Number of pages	13
Journal	Journal of Cereal Science
Volume	11
Issue number	2
DOIs	https://doi.org/10.1016/S0733-5210(09)80113-6
Publication status	Published - 1990
Externally published	Yes

Access to Document

10.1016/S0733-5210(09)80113-6

Cite this

@article{303e959bc15a46dba99b38bf9ea295e7,

title = "Characterization of a panel of monoclonal anti-gliadin antibodies",

abstract = "A panel of monoclonal antibodies has been raised against a total gliadin fraction prepared from the hard bread wheat cultivar Avalon. The antibodies have been characterized by enzyme-linked immunosorhent assay and immunoblotting techniques for their interactions with a range of cereal prolamins, purified gliadins and short synthetic peptides. The antibodies showed broad, intermediate and narrow specificities. The immunological approach offers the protein chemist an additional means of studying the structures and interrelationships of cereal proteins.",

author = "{Clare Mills}, {E. N.} and Burgess, {Shirley R.} and Tatham, {Arthur S.} and Shewry, {Peter R.} and Chan, {Henry W.S.} and Morgan, {Michael R.A.}",

year = "1990",

doi = "10.1016/S0733-5210(09)80113-6",

language = "English",

volume = "11",

pages = "89--101",

journal = "Journal of Cereal Science",

issn = "0733-5210",

number = "2",

}

TY - JOUR

T1 - Characterization of a panel of monoclonal anti-gliadin antibodies

AU - Clare Mills, E. N.

AU - Burgess, Shirley R.

AU - Tatham, Arthur S.

AU - Shewry, Peter R.

AU - Chan, Henry W.S.

AU - Morgan, Michael R.A.

PY - 1990

Y1 - 1990

N2 - A panel of monoclonal antibodies has been raised against a total gliadin fraction prepared from the hard bread wheat cultivar Avalon. The antibodies have been characterized by enzyme-linked immunosorhent assay and immunoblotting techniques for their interactions with a range of cereal prolamins, purified gliadins and short synthetic peptides. The antibodies showed broad, intermediate and narrow specificities. The immunological approach offers the protein chemist an additional means of studying the structures and interrelationships of cereal proteins.

AB - A panel of monoclonal antibodies has been raised against a total gliadin fraction prepared from the hard bread wheat cultivar Avalon. The antibodies have been characterized by enzyme-linked immunosorhent assay and immunoblotting techniques for their interactions with a range of cereal prolamins, purified gliadins and short synthetic peptides. The antibodies showed broad, intermediate and narrow specificities. The immunological approach offers the protein chemist an additional means of studying the structures and interrelationships of cereal proteins.

UR - http://www.scopus.com/inward/record.url?scp=84988145512&partnerID=8YFLogxK

U2 - 10.1016/S0733-5210(09)80113-6

DO - 10.1016/S0733-5210(09)80113-6

M3 - Article

AN - SCOPUS:84988145512

SN - 0733-5210

VL - 11

SP - 89

EP - 101

JO - Journal of Cereal Science

JF - Journal of Cereal Science

IS - 2

ER -

Characterization of a panel of monoclonal anti-gliadin antibodies

Abstract

Access to Document

Other files and links

Cite this