TY - JOUR
T1 - Capillary cortisol sampling during high-intensity exercise
AU - Fryer, S.
AU - Hillier, S.
AU - Dickson, T.
AU - Draper, N.
AU - Stoner, L.
AU - Winter, D.
AU - Young, J.
AU - Cohen, L.
PY - 2012
Y1 - 2012
N2 - Venepuncture is the established "gold standardo" for sampling cortisol, but it is expensive, highly invasive and impractical for many experimental and clinical settings. Salivary free cortisol is a non-invasive and practical alternative; however, when cortisol concentrations exceed 500nmol·L there is a lack of agreement between salivary (free) and venous (bound) cortisol. No known research has assessed whether capillary cortisol accurately reflects venous blood cortisol across a range of concentrations. The objective of the current study was to determine the agreement between capillary and venous blood samples of total plasma cortisol across a range of concentrations. 11 healthy male subjects (26.1±5.3years) were recruited. Capillary and venous blood samples were collected pre and post (immediately post and post 5, 10, 15 and 20min) a treadmill VOtest. Regression analysis revealed a strong relationship (R 2=0.96, y=1.0028x+1.2964 (P<0.05)) between capillary and venous cortisol concentrations. A Bland-Altman plot showed all data was within the upper and lower bounds of the 95% confidence interval, and no systematic bias was evident. In conclusion, capillary sampling is a valid technique for measuring bound cortisol across a range of concentrations.
AB - Venepuncture is the established "gold standardo" for sampling cortisol, but it is expensive, highly invasive and impractical for many experimental and clinical settings. Salivary free cortisol is a non-invasive and practical alternative; however, when cortisol concentrations exceed 500nmol·L there is a lack of agreement between salivary (free) and venous (bound) cortisol. No known research has assessed whether capillary cortisol accurately reflects venous blood cortisol across a range of concentrations. The objective of the current study was to determine the agreement between capillary and venous blood samples of total plasma cortisol across a range of concentrations. 11 healthy male subjects (26.1±5.3years) were recruited. Capillary and venous blood samples were collected pre and post (immediately post and post 5, 10, 15 and 20min) a treadmill VOtest. Regression analysis revealed a strong relationship (R 2=0.96, y=1.0028x+1.2964 (P<0.05)) between capillary and venous cortisol concentrations. A Bland-Altman plot showed all data was within the upper and lower bounds of the 95% confidence interval, and no systematic bias was evident. In conclusion, capillary sampling is a valid technique for measuring bound cortisol across a range of concentrations.
KW - capillary
KW - cortisol
KW - high-intensity exercise
KW - validation
UR - http://www.scopus.com/inward/record.url?scp=84866733516&partnerID=8YFLogxK
U2 - 10.1055/s-0032-1311584
DO - 10.1055/s-0032-1311584
M3 - Article
C2 - 22592547
AN - SCOPUS:84866733516
SN - 0172-4622
VL - 33
SP - 842
EP - 845
JO - International Journal of Sports Medicine
JF - International Journal of Sports Medicine
IS - 10
ER -