Transformation of pasta wheat (Triticum turgidum L. var. durum) with high-molecular-weight glutenin subunit genes and modification of dough functionality

G. Y. He, L. Rooke, S. Steele, F. Békés, P. Gras, A. S. Tatham, R. Fido, P. Barcelo, P. R. Shewry, P. A. Lazzeri*

*Awdur cyfatebol y gwaith hwn

Allbwn ymchwil: Cyfraniad at gyfnodolynErthygladolygiad gan gymheiriaid

104 Dyfyniadau (Scopus)

Crynodeb

Particle bombardment has been used to transform three cultivars (L35, Ofanto, Svevo) and one breeding line (Latino x Lira) of durum wheat (Triticum turgidum L. var. durum). These varieties were co-transformed with plasmids containing selectable and scorable marker genes (bar and uidA) and plasmids containing one of two high-molecular-weight (HMW) glutenin subunit genes (encoding subunits 1Ax1 or 1Dx5). Ten independent transgenic lines were recovered from 1683 bombarded scutella (transformation efficiency thus 0.6%). Five lines expressed either subunit 1Dx5 or 1Ax1 at levels similar to those of endogenous subunits encoded on chromosome 1B. To identify the effects of the transgenes on the functional properties of grain, three lines showing segregation for transgene expression were used to isolate sibling T2 plants which were null or positive for the transgene product. Analysis of these plants using a small-scale mixograph showed that expression of the additional subunits resulted in increased dough strength and stability, demonstrating that transformation can be used to modify the quality of durum wheat for bread and pasta making.

Iaith wreiddiolSaesneg
Tudalennau (o-i)377-386
Nifer y tudalennau10
CyfnodolynMolecular Breeding
Cyfrol5
Rhif cyhoeddi4
Dynodwyr Gwrthrych Digidol (DOIs)
StatwsCyhoeddwyd - 1999
Cyhoeddwyd yn allanolIe

Dyfynnu hyn