The measurement of nitric oxide and its metabolites in biological samples by ozone-based chemiluminescence

Andrew G. Pinder*, Stephen C. Rogers, Afshin Khalatbari, Thomas E. Ingram, Philip E. James

*Awdur cyfatebol y gwaith hwn

Allbwn ymchwil: Pennod mewn Llyfr/Adroddiad/Trafodion CynhadleddPennodadolygiad gan gymheiriaid

40 Dyfyniadau (Scopus)

Crynodeb

A plethora of publications on techniques and methodologies for measuring nitric oxide (NO) or reaction products of NO (NO metabolites) has served in recent years to complicate and confuse the majority of researchers interested in this field. Here, we provide a practical approach and summarize the key issues and corresponding solutions regarding quantification with the use of ozone-based chemiluminescence, which is the most accurate, sensitive, and widely used NO detection method. We have drawn on the vast experience of leaders in the field to produce this consensus, but the views and implications presented herein represent our own, and we limit our advice to those techniques with which we have direct experience. Hopefully, this guide will allow authors to make more informed decisions regarding NO metabolite measurement methodology, without the need for each subsequent group to rediscover previously observed advantages and pitfalls.

Iaith wreiddiolSaesneg
TeitlRedox-Mediated Signal Transduction
Is-deitlMethods and Protocols
GolygyddionJohn Hancock
Tudalennau11-28
Nifer y tudalennau18
Dynodwyr Gwrthrych Digidol (DOIs)
StatwsCyhoeddwyd - 2008
Cyhoeddwyd yn allanolIe

Cyfres gyhoeddiadau

EnwMethods in Molecular Biology
Cyfrol476
ISSN (Argraffiad)1064-3745

Dyfynnu hyn