Molecular weights of high molecular weight subunits of glutenin determined by mass spectrometry

Dominic R. Hickman; Peter Roepstorff; Peter R. Shewry; Arthur S. Tatham

doi:10.1016/0733-5210(95)90038-1

Molecular weights of high molecular weight subunits of glutenin determined by mass spectrometry

Dominic R. Hickman^*, Peter Roepstorff, Peter R. Shewry, Arthur S. Tatham

^*Awdur cyfatebol y gwaith hwn

Allbwn ymchwil: Cyfraniad at gyfnodolyn › Erthygl › adolygiad gan gymheiriaid

51 Dyfyniadau (Scopus)

Crynodeb

High molecular weight (HMW) subunits of glutenin, corresponding to those for which full length gene sequences are available, were purified and their M_rs determined by mass spectrometry [matrix-assisted laser desorption ionisation (MALDI)]. In general, the M_rs determined were within the experimental error of the techniques employed [at the mass range of the high molecular weight (hmw) prolamins, error ∼ 500 Da]. Two subunits, 1 Dx2 and 1By9, however, showed M_rs approximately 900 Da higher and 1700 Da lower, respectively, than expected from their gene sequences. These data confirm that the HMW subunits are not extensively glycosylated and also demonstrate the utility of mass spectrometry for determining the M_rs of prolamins.

Iaith wreiddiol	Saesneg
Tudalennau (o-i)	99-103
Nifer y tudalennau	5
Cyfnodolyn	Journal of Cereal Science
Cyfrol	22
Rhif cyhoeddi	2
Dynodwyr Gwrthrych Digidol (DOIs)	https://doi.org/10.1016/0733-5210(95)90038-1
Statws	Cyhoeddwyd - 1995
Cyhoeddwyd yn allanol	Ie

Mynediad at Ddogfen

10.1016/0733-5210(95)90038-1

Ffeiliau eraill a chysylltiadau

Dolen i’r cyhoeddiad yn Scopus

Dyfynnu hyn

@article{19745db4edd94952929cb1cc86145544,

title = "Molecular weights of high molecular weight subunits of glutenin determined by mass spectrometry",

abstract = "High molecular weight (HMW) subunits of glutenin, corresponding to those for which full length gene sequences are available, were purified and their Mrs determined by mass spectrometry [matrix-assisted laser desorption ionisation (MALDI)]. In general, the Mrs determined were within the experimental error of the techniques employed [at the mass range of the high molecular weight (hmw) prolamins, error ∼ 500 Da]. Two subunits, 1 Dx2 and 1By9, however, showed Mrs approximately 900 Da higher and 1700 Da lower, respectively, than expected from their gene sequences. These data confirm that the HMW subunits are not extensively glycosylated and also demonstrate the utility of mass spectrometry for determining the Mrs of prolamins.",

keywords = "glutenin subunits, mass spectrometry, molecular weights",

author = "Hickman, {Dominic R.} and Peter Roepstorff and Shewry, {Peter R.} and Tatham, {Arthur S.}",

year = "1995",

doi = "10.1016/0733-5210(95)90038-1",

language = "English",

volume = "22",

pages = "99--103",

journal = "Journal of Cereal Science",

issn = "0733-5210",

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}

TY - JOUR

T1 - Molecular weights of high molecular weight subunits of glutenin determined by mass spectrometry

AU - Hickman, Dominic R.

AU - Roepstorff, Peter

AU - Shewry, Peter R.

AU - Tatham, Arthur S.

PY - 1995

Y1 - 1995

N2 - High molecular weight (HMW) subunits of glutenin, corresponding to those for which full length gene sequences are available, were purified and their Mrs determined by mass spectrometry [matrix-assisted laser desorption ionisation (MALDI)]. In general, the Mrs determined were within the experimental error of the techniques employed [at the mass range of the high molecular weight (hmw) prolamins, error ∼ 500 Da]. Two subunits, 1 Dx2 and 1By9, however, showed Mrs approximately 900 Da higher and 1700 Da lower, respectively, than expected from their gene sequences. These data confirm that the HMW subunits are not extensively glycosylated and also demonstrate the utility of mass spectrometry for determining the Mrs of prolamins.

AB - High molecular weight (HMW) subunits of glutenin, corresponding to those for which full length gene sequences are available, were purified and their Mrs determined by mass spectrometry [matrix-assisted laser desorption ionisation (MALDI)]. In general, the Mrs determined were within the experimental error of the techniques employed [at the mass range of the high molecular weight (hmw) prolamins, error ∼ 500 Da]. Two subunits, 1 Dx2 and 1By9, however, showed Mrs approximately 900 Da higher and 1700 Da lower, respectively, than expected from their gene sequences. These data confirm that the HMW subunits are not extensively glycosylated and also demonstrate the utility of mass spectrometry for determining the Mrs of prolamins.

KW - glutenin subunits

KW - mass spectrometry

KW - molecular weights

UR - http://www.scopus.com/inward/record.url?scp=0001589833&partnerID=8YFLogxK

U2 - 10.1016/0733-5210(95)90038-1

DO - 10.1016/0733-5210(95)90038-1

M3 - Article

AN - SCOPUS:0001589833

SN - 0733-5210

VL - 22

SP - 99

EP - 103

JO - Journal of Cereal Science

JF - Journal of Cereal Science

IS - 2

ER -