TY - JOUR
T1 - Corallococcus senghenyddensis sp. nov., a myxobacterium with potent antimicrobial activity
AU - Arakal, Benita S
AU - Rowlands, Richard S
AU - McCarthy, Michael
AU - Whitworth, David E
AU - Maddocks, Sarah E
AU - James, Philip E
AU - Livingstone, Paul G
N1 - © The Author(s) 2024. Published by Oxford University Press on behalf of Applied Microbiology International.
PY - 2024/4/22
Y1 - 2024/4/22
N2 - Aim: Corallococcus species are diverse in the natural environment with 10 new Corallococcus species having been characterized in just the last 5 years. As well as being an abundant myxobacterial genus, they produce several secondary metabolites, including Corallopyronin, Corramycin, Coralmycin, and Corallorazine. We isolated a novel strain Corallococcus spp RDP092CA from soil in South Wales, UK, using Candida albicans as prey bait and characterized its predatory activities against pathogenic bacteria and yeast. Methods and results: The size of the RDP092CA genome was 8.5 Mb with a G + C content of 71.4%. Phylogenetically, RDP092CA is closely related to Corallococcus interemptor, C. coralloides, and C. exiguus. However, genome average nucleotide identity and digital DNA–DNA hybridization values are lower than 95% and 70% when compared to those type strains, implying that it belongs to a novel species. The RDP092CA genome harbours seven types of biosynthetic gene clusters (BGCs) and 152 predicted antimicrobial peptides. In predation assays, RDP092CA showed good predatory activity against Escherichia coli, Pseudomonas aeruginosa, Citrobacter freundii, and Staphylococcus aureus but not against Enterococcus faecalis. It also showed good antibiofilm activity against all five bacteria in biofilm assays. Antifungal activity against eight Candida spp. was variable, with particularly good activity against Meyerozyma guillermondii DSM 6381. Antimicrobial peptide RDP092CA_120 exhibited potent antibiofilm activity with >50% inhibition and >60% dispersion of biofilms at concentrations down to 1 μg/ml. Conclusions: We propose that strain RDP092CA represents a novel species with promising antimicrobial activities, Corallococcus senghenyddensis sp. nov. (=NBRC 116490 T =CCOS 2109 T), based on morphological, biochemical, and genomic features.
AB - Aim: Corallococcus species are diverse in the natural environment with 10 new Corallococcus species having been characterized in just the last 5 years. As well as being an abundant myxobacterial genus, they produce several secondary metabolites, including Corallopyronin, Corramycin, Coralmycin, and Corallorazine. We isolated a novel strain Corallococcus spp RDP092CA from soil in South Wales, UK, using Candida albicans as prey bait and characterized its predatory activities against pathogenic bacteria and yeast. Methods and results: The size of the RDP092CA genome was 8.5 Mb with a G + C content of 71.4%. Phylogenetically, RDP092CA is closely related to Corallococcus interemptor, C. coralloides, and C. exiguus. However, genome average nucleotide identity and digital DNA–DNA hybridization values are lower than 95% and 70% when compared to those type strains, implying that it belongs to a novel species. The RDP092CA genome harbours seven types of biosynthetic gene clusters (BGCs) and 152 predicted antimicrobial peptides. In predation assays, RDP092CA showed good predatory activity against Escherichia coli, Pseudomonas aeruginosa, Citrobacter freundii, and Staphylococcus aureus but not against Enterococcus faecalis. It also showed good antibiofilm activity against all five bacteria in biofilm assays. Antifungal activity against eight Candida spp. was variable, with particularly good activity against Meyerozyma guillermondii DSM 6381. Antimicrobial peptide RDP092CA_120 exhibited potent antibiofilm activity with >50% inhibition and >60% dispersion of biofilms at concentrations down to 1 μg/ml. Conclusions: We propose that strain RDP092CA represents a novel species with promising antimicrobial activities, Corallococcus senghenyddensis sp. nov. (=NBRC 116490 T =CCOS 2109 T), based on morphological, biochemical, and genomic features.
KW - Corallococcus
KW - antimicrobial peptide
KW - biofilm
KW - genome sequences
KW - predation
KW - Anti-Infective Agents/pharmacology
KW - Genome, Bacterial
KW - Multigene Family
KW - Soil Microbiology
KW - RNA, Ribosomal, 16S/genetics
KW - Candida albicans/drug effects
KW - Phylogeny
KW - Microbial Sensitivity Tests
KW - Base Composition
KW - Myxococcales/genetics
KW - DNA, Bacterial/genetics
UR - http://www.scopus.com/inward/record.url?scp=85193307864&partnerID=8YFLogxK
U2 - 10.1093/jambio/lxae102
DO - 10.1093/jambio/lxae102
M3 - Article
C2 - 38649930
SN - 1364-5072
VL - 135
JO - Journal of Applied Microbiology
JF - Journal of Applied Microbiology
IS - 5
M1 - lxae102
ER -