TY - JOUR
T1 - Filterability of leukocytes in purified suspensions and in diluted whole blood
AU - Kooshesh, F.
AU - Evans, S. A.
AU - Jones, J. G.
AU - Wardrop, C. A.J.
PY - 1991
Y1 - 1991
N2 - The transit time of adult leukocytes through 5 μm Nuclepore membranes has been calculated for subpopulations both in isolation and in co-suspensions. The results agree with previous findings that granulocytes and lymphocytes have similar properties. In suspensions of unfractionated leukocytes these subpopulations can not be distinguished and have a mean transit time of 1.17±0.11 s at 5 cm H2O pressure. In all cases the number of pore-blocking cells corresponds approximately to the number of monocytes, being 1.1%, 11.8% and 4.8% respectively in the granulocyte, mononuclear and unfractionated suspensions. The transit time of purified red cells and the mean transit time for the majority of leukocytes, calculated for diluted but unfractionated blood, are 1.21±0.08 ms and 0.85±0.06 s respectively at a pressure of 10 cm H2O. Under the same conditions, the mean transit time for isolated but unfractionated leukocytes is 0.51±0.06 s. Density centrifugation therefore seems to be an acceptable, but not essential, prerequisite to studying the filterability of human leukocytes.
AB - The transit time of adult leukocytes through 5 μm Nuclepore membranes has been calculated for subpopulations both in isolation and in co-suspensions. The results agree with previous findings that granulocytes and lymphocytes have similar properties. In suspensions of unfractionated leukocytes these subpopulations can not be distinguished and have a mean transit time of 1.17±0.11 s at 5 cm H2O pressure. In all cases the number of pore-blocking cells corresponds approximately to the number of monocytes, being 1.1%, 11.8% and 4.8% respectively in the granulocyte, mononuclear and unfractionated suspensions. The transit time of purified red cells and the mean transit time for the majority of leukocytes, calculated for diluted but unfractionated blood, are 1.21±0.08 ms and 0.85±0.06 s respectively at a pressure of 10 cm H2O. Under the same conditions, the mean transit time for isolated but unfractionated leukocytes is 0.51±0.06 s. Density centrifugation therefore seems to be an acceptable, but not essential, prerequisite to studying the filterability of human leukocytes.
KW - Blood rheology
KW - Erythrocyte deformability
KW - Leukocyte deformability
UR - http://www.scopus.com/inward/record.url?scp=0025731828&partnerID=8YFLogxK
U2 - 10.3233/CH-1991-111-210
DO - 10.3233/CH-1991-111-210
M3 - Article
AN - SCOPUS:0025731828
SN - 1386-0291
VL - 11
SP - 79
EP - 89
JO - Clinical Hemorheology and Microcirculation
JF - Clinical Hemorheology and Microcirculation
IS - 1-2
ER -