Control of immune ligands by members of a cytomegalovirus gene expansion suppresses natural killer cell activation

Ceri A. Fielding; Michael P. Weekes; Luis V. Nobre; Eva Ruckova; Gavin S. Wilkie; Joao A. Paulo; Chiwen Chang; Nicolás M. Suárez; James A. Davies; Robin Antrobus; Richard J. Stanton; Rebecca J. Aicheler; Hester Nichols; Borek Vojtesek; John Trowsdale; Andrew J. Davison; Steven P. Gygi; Peter Tomasec; Paul J. Lehner; Gavin W.G. Wilkinson

doi:10.7554/eLife.22206

Control of immune ligands by members of a cytomegalovirus gene expansion suppresses natural killer cell activation

Ceri A. Fielding, Michael P. Weekes, Luis V. Nobre, Eva Ruckova, Gavin S. Wilkie, Joao A. Paulo, Chiwen Chang, Nicolás M. Suárez, James A. Davies, Robin Antrobus, Richard J. Stanton, Rebecca J. Aicheler, Hester Nichols, Borek Vojtesek, John Trowsdale, Andrew J. Davison, Steven P. Gygi, Peter Tomasec, Paul J. Lehner, Gavin W.G. Wilkinson^*

^*Awdur cyfatebol y gwaith hwn

Allbwn ymchwil: Cyfraniad at gyfnodolyn › Erthygl › adolygiad gan gymheiriaid

61 Dyfyniadau (Scopus)

Crynodeb

The human cytomegalovirus (HCMV) US12 family consists of ten sequentially arranged genes (US12-21) with poorly characterized function. We now identify novel natural killer (NK) cell evasion functions for four members: US12, US14, US18 and US20. Using a systematic multiplexed proteomics approach to quantify ~1300 cell surface and ~7200 whole cell proteins, we demonstrate that the US12 family selectively targets plasma membrane proteins and plays key roles in regulating NK ligands, adhesion molecules and cytokine receptors. US18 and US20 work in concert to suppress cell surface expression of the critical NKp30 ligand B7-H6 thus inhibiting NK cell activation. The US12 family is therefore identified as a major new hub of immune regulation.

Iaith wreiddiol	Saesneg
Rhif yr erthygl	e22206
Cyfnodolyn	eLife
Cyfrol	6
Dynodwyr Gwrthrych Digidol (DOIs)	https://doi.org/10.7554/eLife.22206
Statws	Cyhoeddwyd - 10 Chwef 2017

Mynediad at Ddogfen

10.7554/eLife.22206

Ffeiliau eraill a chysylltiadau

Dolen i’r cyhoeddiad yn Scopus

Dyfynnu hyn

Fielding, C. A., Weekes, M. P., Nobre, L. V., Ruckova, E., Wilkie, G. S., Paulo, J. A., Chang, C., Suárez, N. M., Davies, J. A., Antrobus, R., Stanton, R. J., Aicheler, R. J., Nichols, H., Vojtesek, B., Trowsdale, J., Davison, A. J., Gygi, S. P., Tomasec, P., Lehner, P. J., & Wilkinson, G. W. G. (2017). Control of immune ligands by members of a cytomegalovirus gene expansion suppresses natural killer cell activation. eLife, 6, erthygl e22206. https://doi.org/10.7554/eLife.22206

@article{142df143041949d6a9025ffb777b3f04,

title = "Control of immune ligands by members of a cytomegalovirus gene expansion suppresses natural killer cell activation",

abstract = "The human cytomegalovirus (HCMV) US12 family consists of ten sequentially arranged genes (US12-21) with poorly characterized function. We now identify novel natural killer (NK) cell evasion functions for four members: US12, US14, US18 and US20. Using a systematic multiplexed proteomics approach to quantify ~1300 cell surface and ~7200 whole cell proteins, we demonstrate that the US12 family selectively targets plasma membrane proteins and plays key roles in regulating NK ligands, adhesion molecules and cytokine receptors. US18 and US20 work in concert to suppress cell surface expression of the critical NKp30 ligand B7-H6 thus inhibiting NK cell activation. The US12 family is therefore identified as a major new hub of immune regulation.",

author = "Fielding, {Ceri A.} and Weekes, {Michael P.} and Nobre, {Luis V.} and Eva Ruckova and Wilkie, {Gavin S.} and Paulo, {Joao A.} and Chiwen Chang and Su{\'a}rez, {Nicol{\'a}s M.} and Davies, {James A.} and Robin Antrobus and Stanton, {Richard J.} and Aicheler, {Rebecca J.} and Hester Nichols and Borek Vojtesek and John Trowsdale and Davison, {Andrew J.} and Gygi, {Steven P.} and Peter Tomasec and Lehner, {Paul J.} and Wilkinson, {Gavin W.G.}",

note = "Publisher Copyright: {\textcopyright} Fielding et al.",

year = "2017",

month = feb,

day = "10",

doi = "10.7554/eLife.22206",

language = "English",

volume = "6",

journal = "eLife",

issn = "2050-084X",

}

Fielding, CA, Weekes, MP, Nobre, LV, Ruckova, E, Wilkie, GS, Paulo, JA, Chang, C, Suárez, NM, Davies, JA, Antrobus, R, Stanton, RJ, Aicheler, RJ, Nichols, H, Vojtesek, B, Trowsdale, J, Davison, AJ, Gygi, SP, Tomasec, P, Lehner, PJ & Wilkinson, GWG 2017, 'Control of immune ligands by members of a cytomegalovirus gene expansion suppresses natural killer cell activation', eLife, cyfrol. 6, e22206. https://doi.org/10.7554/eLife.22206

TY - JOUR

T1 - Control of immune ligands by members of a cytomegalovirus gene expansion suppresses natural killer cell activation

AU - Fielding, Ceri A.

AU - Weekes, Michael P.

AU - Nobre, Luis V.

AU - Ruckova, Eva

AU - Wilkie, Gavin S.

AU - Paulo, Joao A.

AU - Chang, Chiwen

AU - Suárez, Nicolás M.

AU - Davies, James A.

AU - Antrobus, Robin

AU - Stanton, Richard J.

AU - Aicheler, Rebecca J.

AU - Nichols, Hester

AU - Vojtesek, Borek

AU - Trowsdale, John

AU - Davison, Andrew J.

AU - Gygi, Steven P.

AU - Tomasec, Peter

AU - Lehner, Paul J.

AU - Wilkinson, Gavin W.G.

PY - 2017/2/10

Y1 - 2017/2/10

N2 - The human cytomegalovirus (HCMV) US12 family consists of ten sequentially arranged genes (US12-21) with poorly characterized function. We now identify novel natural killer (NK) cell evasion functions for four members: US12, US14, US18 and US20. Using a systematic multiplexed proteomics approach to quantify ~1300 cell surface and ~7200 whole cell proteins, we demonstrate that the US12 family selectively targets plasma membrane proteins and plays key roles in regulating NK ligands, adhesion molecules and cytokine receptors. US18 and US20 work in concert to suppress cell surface expression of the critical NKp30 ligand B7-H6 thus inhibiting NK cell activation. The US12 family is therefore identified as a major new hub of immune regulation.

AB - The human cytomegalovirus (HCMV) US12 family consists of ten sequentially arranged genes (US12-21) with poorly characterized function. We now identify novel natural killer (NK) cell evasion functions for four members: US12, US14, US18 and US20. Using a systematic multiplexed proteomics approach to quantify ~1300 cell surface and ~7200 whole cell proteins, we demonstrate that the US12 family selectively targets plasma membrane proteins and plays key roles in regulating NK ligands, adhesion molecules and cytokine receptors. US18 and US20 work in concert to suppress cell surface expression of the critical NKp30 ligand B7-H6 thus inhibiting NK cell activation. The US12 family is therefore identified as a major new hub of immune regulation.

UR - http://www.scopus.com/inward/record.url?scp=85016333839&partnerID=8YFLogxK

U2 - 10.7554/eLife.22206

DO - 10.7554/eLife.22206

M3 - Article

C2 - 28186488

AN - SCOPUS:85016333839

SN - 2050-084X

VL - 6

JO - eLife

JF - eLife

M1 - e22206

ER -