Characterisation of a novel high Mr glutenin subunit encoded by chromosome 1D of bread wheat

F. Buonocore; D. R. Hickman; C. Caporale; E. Porceddu; D. Lafiandra; A. S. Tatham; P. R. Shewry

doi:10.1006/jcrs.1996.0005

Characterisation of a novel high M_r glutenin subunit encoded by chromosome 1D of bread wheat

F. Buonocore^*
, D. R. Hickman
, C. Caporale
, E. Porceddu
, D. Lafiandra
, A. S. Tatham
, P. R. Shewry

^*Awdur cyfatebol y gwaith hwn

Allbwn ymchwil: Cyfraniad at gyfnodolyn › Erthygl › adolygiad gan gymheiriaid

9 Dyfyniadau (Scopus)

Crynodeb

A high M_r subunit of wheat glutenin (1Dx2·2*), having an unusually high M_r (approximately 110000), was purified using a combination of preparative isoelectric focusing and reversed-phase high performance liquid chromatography. Comparison of its N-terminal sequence and amino acid composition with those of the allelic subunit 1Dx2 indicate that its greater M_r could be due to the presence of a larger central repetitive domain. Spectrofluorimetric and circular dichroism analyses suggest that an increase in the length of this domain does not affect the conformational properties of the subunit.

Iaith wreiddiol	Saesneg
Tudalennau (o-i)	55-60
Nifer y tudalennau	6
Cyfnodolyn	Journal of Cereal Science
Cyfrol	23
Rhif cyhoeddi	1
Dynodwyr Gwrthrych Digidol (DOIs)	https://doi.org/10.1006/jcrs.1996.0005
Statws	Cyhoeddwyd - Ion 1996
Cyhoeddwyd yn allanol	Ie

Mynediad at Ddogfen

10.1006/jcrs.1996.0005

Ffeiliau eraill a chysylltiadau

Dolen i’r cyhoeddiad yn Scopus

Dyfynnu hyn

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title = "Characterisation of a novel high Mr glutenin subunit encoded by chromosome 1D of bread wheat",

abstract = "A high Mr subunit of wheat glutenin (1Dx2·2*), having an unusually high Mr (approximately 110000), was purified using a combination of preparative isoelectric focusing and reversed-phase high performance liquid chromatography. Comparison of its N-terminal sequence and amino acid composition with those of the allelic subunit 1Dx2 indicate that its greater Mr could be due to the presence of a larger central repetitive domain. Spectrofluorimetric and circular dichroism analyses suggest that an increase in the length of this domain does not affect the conformational properties of the subunit.",

keywords = "High M glutenin subunits",

author = "F. Buonocore and Hickman, \{D. R.\} and C. Caporale and E. Porceddu and D. Lafiandra and Tatham, \{A. S.\} and Shewry, \{P. R.\}",

year = "1996",

month = jan,

doi = "10.1006/jcrs.1996.0005",

language = "English",

volume = "23",

pages = "55--60",

journal = "Journal of Cereal Science",

issn = "0733-5210",

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TY - JOUR

T1 - Characterisation of a novel high Mr glutenin subunit encoded by chromosome 1D of bread wheat

AU - Buonocore, F.

AU - Hickman, D. R.

AU - Caporale, C.

AU - Porceddu, E.

AU - Lafiandra, D.

AU - Tatham, A. S.

AU - Shewry, P. R.

PY - 1996/1

Y1 - 1996/1

N2 - A high Mr subunit of wheat glutenin (1Dx2·2*), having an unusually high Mr (approximately 110000), was purified using a combination of preparative isoelectric focusing and reversed-phase high performance liquid chromatography. Comparison of its N-terminal sequence and amino acid composition with those of the allelic subunit 1Dx2 indicate that its greater Mr could be due to the presence of a larger central repetitive domain. Spectrofluorimetric and circular dichroism analyses suggest that an increase in the length of this domain does not affect the conformational properties of the subunit.

AB - A high Mr subunit of wheat glutenin (1Dx2·2*), having an unusually high Mr (approximately 110000), was purified using a combination of preparative isoelectric focusing and reversed-phase high performance liquid chromatography. Comparison of its N-terminal sequence and amino acid composition with those of the allelic subunit 1Dx2 indicate that its greater Mr could be due to the presence of a larger central repetitive domain. Spectrofluorimetric and circular dichroism analyses suggest that an increase in the length of this domain does not affect the conformational properties of the subunit.

KW - High M glutenin subunits

UR - https://www.scopus.com/pages/publications/0001849608

U2 - 10.1006/jcrs.1996.0005

DO - 10.1006/jcrs.1996.0005

M3 - Article

AN - SCOPUS:0001849608

SN - 0733-5210

VL - 23

SP - 55

EP - 60

JO - Journal of Cereal Science

JF - Journal of Cereal Science

IS - 1

ER -