Adenovirus vector delivery stimulates natural killer cell recognition

Peter Tomasec, Eddie C.Y. Wang, Veronika Groh, Thomas Spies, Brian P. McSharry, Rebecca J. Aicheler, Richard J. Stanton, Gavin W.G. Wilkinson*

*Awdur cyfatebol y gwaith hwn

Allbwn ymchwil: Cyfraniad at gyfnodolynErthygladolygiad gan gymheiriaid

16 Dyfyniadau (Scopus)

Crynodeb

We report that delivery of first-generation replication-deficient adenovirus (RDAd) vectors into primary human fibroblasts is associated with the induction of natural killer (NK) cell-mediated cytolysis in vitro. RDAd vector delivery induced cytolysis by a range of NK cell populations including the NK cell clone NKL, primary polyclonal NK lines and a proportion of NK clones (36 %) in autologous HLA-matched assays. Adenovirus-induced cytolysis was inhibited by antibody blocking of the NK-activating receptor NKG2D, implicating this receptor in this function. NKG2D is ubiquitously expressed on NK cells and CD8+ T cells. Significantly, γ-irradiation of the vector eliminated the effect, suggesting that breakthrough expression from the vector induces at least some of the pro-inflammatory responses of unknown aetiology following the application of RDAd vectors during in vivo gene delivery.

Iaith wreiddiolSaesneg
Tudalennau (o-i)1103-1108
Nifer y tudalennau6
CyfnodolynJournal of General Virology
Cyfrol88
Rhif cyhoeddi4
Dynodwyr Gwrthrych Digidol (DOIs)
StatwsCyhoeddwyd - 1 Ebr 2007
Cyhoeddwyd yn allanolIe

Dyfynnu hyn